Aluminium in PDB 1fqj: Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

Enzymatic activity of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

All present enzymatic activity of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]:
3.1.4.35;

Protein crystallography data

The structure of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)], PDB code: 1fqj was solved by K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	50.00 / 2.02
*Space group*	P 2₁ 2₁ 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	91.609, 115.937, 134.188, 90.00, 90.00, 90.00
*R / R_free (%)*	23.3 / 26.5

Other elements in 1fqj:

Fluorine	(F)	8 atoms
Magnesium	(Mg)	2 atoms

Aluminium Binding Sites:

The binding sites of Aluminium atom in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] (pdb code 1fqj). This binding sites where shown within 5.0 Angstroms radius around Aluminium atom.
In total 2 binding sites of Aluminium where determined in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)], PDB code: 1fqj:
Jump to Aluminium binding site number: 1; 2;

Aluminium binding site 1 out of 2 in 1fqj

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Aluminium Binding Sites List in 1fqj

Aluminium binding site 1 out of 2 in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

Mono view

Stereo pair view

A full contact list of Aluminium with other atoms in the Al binding site number 1 of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Al362 b:17.7 occ:1.00	AL	A:ALF362	0.0	17.7	1.0
	F2	A:ALF362	1.8	16.4	1.0
	F4	A:ALF362	1.8	21.6	1.0
	F1	A:ALF362	1.8	25.1	1.0
	F3	A:ALF362	1.8	22.9	1.0
	O	A:HOH363	2.3	12.2	1.0
	O3B	A:GDP360	2.4	15.2	1.0
	PB	A:GDP360	3.3	15.0	1.0
	O2B	A:GDP360	3.4	10.4	1.0
	MG	A:MG352	3.4	17.1	1.0
	NH1	A:ARG174	3.7	7.9	1.0
	N	A:GLU39	3.8	19.2	1.0
	O	A:HOH364	3.9	10.1	1.0
	NZ	A:LYS42	4.0	10.9	1.0
	N	A:GLY199	4.0	13.8	1.0
	O1B	A:GDP360	4.0	16.2	1.0
	NH2	A:ARG174	4.1	8.4	1.0
	OE1	A:GLN200	4.2	11.7	1.0
	N	A:THR177	4.2	12.1	1.0
	NE2	A:GLN200	4.2	12.6	1.0
	CA	A:GLY38	4.3	14.3	1.0
	O	A:HOH365	4.4	14.3	1.0
	CZ	A:ARG174	4.4	13.4	1.0
	O3A	A:GDP360	4.4	11.6	1.0
	OG1	A:THR177	4.5	20.0	1.0
	CB	A:THR177	4.5	21.9	1.0
	C	A:GLY38	4.6	17.3	1.0
	CA	A:GLU39	4.6	23.6	1.0
	CA	A:GLY198	4.6	21.1	1.0
	CD	A:GLN200	4.6	16.4	1.0
	O	A:THR177	4.7	14.7	1.0
	C	A:GLY198	4.8	18.0	1.0
	CA	A:GLY199	4.8	13.1	1.0
	CA	A:THR177	4.9	14.9	1.0

Aluminium binding site 2 out of 2 in 1fqj

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Aluminium Binding Sites List in 1fqj

Aluminium binding site 2 out of 2 in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

Mono view

Stereo pair view

A full contact list of Aluminium with other atoms in the Al binding site number 2 of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
D:Al363 b:16.5 occ:1.00	AL	D:ALF363	0.0	16.5	1.0
	F1	D:ALF363	1.8	14.0	1.0
	F3	D:ALF363	1.8	20.8	1.0
	F2	D:ALF363	1.8	14.3	1.0
	F4	D:ALF363	1.8	18.8	1.0
	O	D:HOH364	2.0	12.2	1.0
	O3B	D:GDP361	2.7	29.6	1.0
	O2B	D:GDP361	3.3	15.1	1.0
	PB	D:GDP361	3.5	20.4	1.0
	MG	D:MG352	3.5	22.7	1.0
	NH1	D:ARG174	3.8	11.4	1.0
	N	D:GLU39	4.0	13.9	1.0
	N	D:THR177	4.0	10.0	1.0
	NH2	D:ARG174	4.0	18.2	1.0
	O	D:HOH365	4.0	8.4	1.0
	N	D:GLY199	4.1	15.7	1.0
	NE2	D:GLN200	4.1	13.2	1.0
	NZ	D:LYS42	4.1	17.9	1.0
	OE1	D:GLN200	4.1	11.1	1.0
	O	D:HOH366	4.3	15.5	1.0
	O1B	D:GDP361	4.4	15.2	1.0
	CB	D:THR177	4.4	14.4	1.0
	CZ	D:ARG174	4.4	11.1	1.0
	CA	D:GLY38	4.5	20.3	1.0
	O3A	D:GDP361	4.5	15.2	1.0
	CD	D:GLN200	4.6	16.6	1.0
	OG1	D:THR177	4.6	18.4	1.0
	CE	D:LYS42	4.6	17.1	1.0
	O	D:THR177	4.6	13.2	1.0
	CA	D:GLY198	4.6	15.4	1.0
	CA	D:THR177	4.7	15.2	1.0
	CG	D:LYS176	4.7	27.3	1.0
	C	D:GLY38	4.8	17.2	1.0
	CA	D:GLU39	4.8	22.9	1.0
	CA	D:LYS176	4.8	21.9	1.0
	C	D:GLY198	4.8	12.8	1.0
	CA	D:GLY199	4.9	12.3	1.0
	C	D:LYS176	4.9	15.2	1.0
	O2A	D:GDP361	5.0	14.5	1.0
	O	D:VAL197	5.0	28.8	1.0

Reference:

K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler. Structural Determinants For Regulation of Phosphodiesterase By A G Protein at 2.0 A. Nature V. 409 1071 2001.
ISSN: ISSN 0028-0836
PubMed: 11234020
DOI: 10.1038/35059138

Page generated: Wed Jul 10 09:20:41 2024

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