Aluminium in PDB 3zs9: S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment

All present enzymatic activity of S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment:
3.6.3.16;

The structure of S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment, PDB code: 3zs9 was solved by M.Mariappan, A.Mateja, M.Dobosz, E.Bove, R.S.Hegde, R.J.Keenan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.537 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	52.558, 77.317, 165.831, 90.00, 90.00, 90.00
R / Rfree (%)	18.22 / 23.8

The structure of S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Magnesium	(Mg)	2 atoms
Zinc	(Zn)	1 atom

The binding sites of Aluminium atom in the S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment (pdb code 3zs9). This binding sites where shown within 5.0 Angstroms radius around Aluminium atom.
In total 2 binding sites of Aluminium where determined in the S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment, PDB code: 3zs9:
Jump to Aluminium binding site number: 1; 2;

Aluminium binding site 1 out of 2 in the S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment


Mono view


Stereo pair view

A full contact list of Aluminium with other atoms in the Al binding site number 1 of S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment within 5.0Å range: probe atom residue distance (Å) B Occ A:Al402 b:19.7 occ:1.00 AL A:ALF402 0.0 19.7 1.0 F3 A:ALF402 1.8 20.1 1.0 F4 A:ALF402 1.8 42.0 1.0 F2 A:ALF402 1.8 32.8 1.0 F1 A:ALF402 1.8 30.3 1.0 O2B A:ADP401 1.9 12.5 1.0 O A:HOH2035 2.2 10.7 1.0 PB A:ADP401 3.2 14.4 1.0 MG A:MG403 3.3 18.1 1.0 O3B A:ADP401 3.5 16.1 1.0 NZ B:LYS26 3.7 12.3 1.0 OD2 A:ASP57 3.7 16.6 1.0 O A:HOH1403 3.7 12.5 1.0 O A:HOH2036 3.7 19.6 1.0 O A:HOH1402 4.0 24.1 1.0 O1B A:ADP401 4.0 22.2 1.0 N A:GLY28 4.1 14.6 1.0 O B:HOH2016 4.1 19.4 1.0 NZ A:LYS31 4.2 17.1 1.0 O3A A:ADP401 4.3 17.1 1.0 O A:HOH1401 4.3 10.1 1.0 N B:GLY27 4.4 17.8 1.0 CE B:LYS26 4.4 12.1 1.0 CA B:GLY27 4.5 11.8 1.0 CG A:PRO169 4.5 14.7 1.0 CG B:LYS26 4.5 12.4 1.0 CE A:LYS31 4.5 18.9 1.0 OD1 A:ASN61 4.7 25.6 1.0 CA A:GLY28 4.7 13.4 1.0 CD A:PRO169 4.7 16.2 1.0 O2A A:ADP401 4.8 16.9 1.0 CA A:GLY27 4.8 15.6 1.0 CG A:ASP57 4.9 16.8 1.0 C A:GLY27 5.0 16.9 1.0

Aluminium binding site 2 out of 2 in the S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment


Mono view


Stereo pair view

A full contact list of Aluminium with other atoms in the Al binding site number 2 of S. Cerevisiae GET3-Adp-ALF4- Complex with A Cytosolic GET2 Fragment within 5.0Å range: probe atom residue distance (Å) B Occ B:Al402 b:24.7 occ:1.00 AL B:ALF402 0.0 24.7 1.0 F1 B:ALF402 1.8 28.3 1.0 F4 B:ALF402 1.8 31.7 1.0 F2 B:ALF402 1.8 26.2 1.0 F3 B:ALF402 1.8 27.2 1.0 O2B B:ADP401 1.9 13.4 1.0 O B:HOH2029 2.2 18.6 1.0 PB B:ADP401 3.2 14.8 1.0 MG B:MG403 3.3 17.8 1.0 O3B B:ADP401 3.5 12.7 1.0 NZ A:LYS26 3.5 16.0 1.0 O B:HOH1403 3.7 13.6 1.0 O A:HOH2085 3.7 23.0 1.0 OD2 B:ASP57 3.9 19.1 1.0 O1B B:ADP401 4.0 15.8 1.0 N B:GLY28 4.0 19.3 1.0 NZ B:LYS31 4.1 16.8 1.0 O A:HOH2017 4.1 15.0 1.0 O B:HOH1402 4.1 20.4 1.0 O3A B:ADP401 4.2 18.1 1.0 O B:HOH1401 4.3 18.1 1.0 CE A:LYS26 4.3 16.9 1.0 N A:GLY27 4.4 15.9 1.0 CG A:LYS26 4.5 11.4 1.0 CG B:PRO169 4.5 19.1 1.0 CA A:GLY27 4.6 15.6 1.0 CA B:GLY28 4.6 12.0 1.0 CE B:LYS31 4.7 18.9 1.0 O2A B:ADP401 4.7 19.7 1.0 ND2 B:ASN61 4.9 24.9 1.0 CD B:PRO169 4.9 17.4 1.0 CA B:GLY27 4.9 11.8 1.0 C B:GLY27 5.0 14.4 1.0

M.Mariappan, A.Mateja, M.Dobosz, E.Bove, R.S.Hegde, R.J.Keenan. The Mechanism of Membrane-Associated Steps in Tail-Anchored Protein Insertion. Nature V. 477 61 2011.
ISSN: ISSN 0028-0836
PubMed: 21866104
DOI: 10.1038/NATURE10362